Coding
Part:BBa_K4065007
Designed by: Raúl Acosta Murillo Group: iGEM21_FCB-UANL (2021-08-14)
YokF Endonuclease derived from the prophage SPBeta
Endonuclease derived from the prophage SPBeta which catalyzes the hydrolysis of supercoiled DNA and RNA (Uniprot, 2021).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 213
Illegal PstI site found at 57
Illegal PstI site found at 385
Illegal PstI site found at 650 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 57
Illegal PstI site found at 385
Illegal PstI site found at 650 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 213
Illegal PstI site found at 57
Illegal PstI site found at 385
Illegal PstI site found at 650 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 213
Illegal PstI site found at 57
Illegal PstI site found at 385
Illegal PstI site found at 650 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |